Generation of cDNA Libraries. Methods and Protocols
Shao-Yao Ying
Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation.
Категории:
Год:
2010
Издание:
1st Edition.
Издательство:
Humana Press
Язык:
english
Страницы:
331
ISBN 10:
1617373338
ISBN 13:
9781617373336
Серия:
Methods in Molecular Biology
Файл:
PDF, 5.56 MB
IPFS:
,
english, 2010